Journal: Cancer Cell International

Article Title: Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression

doi: 10.1186/s12935-025-04089-y

Figure Lengend Snippet: UNG activated several signaling pathways in CRC cells. Western blot was performed to detect the levels of mTOR, p-mTOR, p70 S6K, p-P70 S6K, AKT, p-AKT, AMPK, p-AMPK, ERK, p-ERK, Bax, Bcl2, cleavages of caspase-9, and caspase-3 in UNG-knockdown CRC cells. Fold changes (Fc) are shown below the bars

Article Snippet: The membranes were incubated overnight at 4 °C with the following primary antibodies: UNG, P70 S6K, p-P70 S6K(S424) (1:1000, Bioworld), β-actin (1:1000, Abcam), cleaved caspase-3, AMPKα1/AMPKα2, p-AMPKα1(Thr183)/AMPKα2(Thr172) (1:1000, Beyotime), BAX, BCL2, AKT1/2/3, mTOR, and p-mTOR (Ser2448) (1:1000; BOSTER), cleaved caspase-9, p-AKT(Ser473), ERK1/2, and p-ERK1/2(Thr202/Tyr204)/(Thr185/Tyr187) (1:1000; ZEN BIO), and GAPDH (1:1000; Proteintech).

Techniques: Protein-Protein interactions, Western Blot, Knockdown

Journal: Journal of Translational Medicine

Article Title: 18-β-glycyrrhetinic acid facilitates nuclear-mitochondrial communications to alleviate oxidative stress through HMGB1-cGAS-Mul1 axis in tendinopathy

doi: 10.1186/s12967-026-08091-4

Figure Lengend Snippet: GA attenuates oxidative stress by inhibiting inflammation to alleviate tendinopathy. In vivo, Wistar rats were divided into 6 groups: vehicle, ciprofloxacin, ciprofloxacin + 25 mg/kg GA, ciprofloxacin + 50 mg/kg GA, ciprofloxacin + 100 mg/kg GA and positive control (five rats in each group). ( A ) H&E and Masson staining were used to observe collagen organization (blue: collagen fibres; red: muscle fibres); Sirius red staining was used to distinguish between type I and type III collagen (scale bars: 10×, 100 μm); ( B ) The expression of collagen I/III was determined by immunofluorescence staining (scale bars: 10×, 100 μm); ( C ) The protein expression of PTGS2 in rat tendon tissues was assessed by immunohistochemical staining (scale bars: 10×, 100 μm). ( D-E ) Biological process and protein function enrichment analysis of the TMT proteomics data. ( F-H ) The serum levels of MDA, GSH and IL-1β were determined with the relevant kits; ( n = 5, data are presented as mean ± SD, ** p < 0.01, *** p < 0.001 vs. vehicle group; ## p < 0.01, ### p < 0.001 vs. Ciprofloxacin group)

Article Snippet: The anti-NLRP3 antibody (#BA3677) and anti-Collagen Type III antibody (#M00788-1) were obtained from Boster, and the anti-ASC antibody (#HA721306) was obtained from Hangzhou Huaan Biotechnology.

Techniques: In Vivo, Positive Control, Staining, Expressing, Immunofluorescence, Immunohistochemical staining

Journal: Journal of Translational Medicine

Article Title: 18-β-glycyrrhetinic acid facilitates nuclear-mitochondrial communications to alleviate oxidative stress through HMGB1-cGAS-Mul1 axis in tendinopathy

doi: 10.1186/s12967-026-08091-4

Figure Lengend Snippet: GA increases TSC viability by inhibiting oxidative stress and inflammatory responses. In vitro, TSCs were pretreated with different concentrations of GA for 12 h and then subjected to the prescribed concentration of H 2 O 2 (100 µM) for 24 h. ( A-B ) Cell viability was assessed by MTT assay; ( C ) Cell viability was assessed by CCK-8 assay; ( D-E ) The levels of GSH and MDA were determined with the relevant kits; ( F ) The content of ROS in TSCs was measured by flow cytometry using DCFH-DA probe; ( G-H ) Western blotting and qPCR were used to assess the protein and gene expression of inflammatory cytokines, including IL-1β, IL-6, and IL-8; ( I ) Tenomodulin, Collagen I and Collagen III protein levels were assessed via western blotting. ( n = 3, data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. H₂O₂ group)

Article Snippet: The anti-NLRP3 antibody (#BA3677) and anti-Collagen Type III antibody (#M00788-1) were obtained from Boster, and the anti-ASC antibody (#HA721306) was obtained from Hangzhou Huaan Biotechnology.

Techniques: In Vitro, Concentration Assay, MTT Assay, CCK-8 Assay, Flow Cytometry, Western Blot, Gene Expression, Control

Journal: Journal of Translational Medicine

Article Title: 18-β-glycyrrhetinic acid facilitates nuclear-mitochondrial communications to alleviate oxidative stress through HMGB1-cGAS-Mul1 axis in tendinopathy

doi: 10.1186/s12967-026-08091-4

Figure Lengend Snippet: GA protects TSCs against oxidative stress via the HMGB1-cGAS-NLRP3 axis in tendinopathy. Wistar rats were divided into five groups (five rats per group): vehicle, ciprofloxacin, ciprofloxacin + 50 mg/kg GA, ciprofloxacin + 50 mg/kg GA + VA-Lip-Ctrl-Plasmid, and ciprofloxacin + 50 mg/kg GA + VA-Lip-cGAS-Plasmid. ( A-B ) Immunohistochemical detection for cGAS, HMGB1, NLRP3 and ASC in tendon tissues (scale bars: 10×, 100 μm); ( C ) H&E and Masson staining were used to observe the collagen organization of tendon tissues (blue indicates collagen fibres and red indicates muscle fibres); Sirius red staining was used to distinguish between type I and type III collagen (Scale bars: 10×, 100 μm); ( D ) The expression of collagen I/III were assessed by immunofluorescence staining (scale bars: 10×, 100 μm); ( E ) Immunohistochemical detection of the expression of NLRP3 and ASC (scale bars: 10×, 100 μm); ( F-H ) Serum levels of MDA and IL-1β were measured by commercial ELISA kits; ( I ) Immunofluorescence staining was used to detect the expression and colocalization of cGAS and HMGB1 in the tendon tissues of the rats (scale bars: 20×, 50 μm). ( J ) Immunofluorescence staining was used to detect the colocalization of cGAS and HMGB1 in the tendon tissue of clinical sample 1. ( n = 5, data are presented as mean ± SD, ** p < 0.01, *** p < 0.001 vs. vehicle group; ## p < 0.01, ### p < 0.001 vs. Ciprofloxacin group; && p < 0.01, &&& p < 0.001 vs. Ciprofloxacin + GA group)

Article Snippet: The anti-NLRP3 antibody (#BA3677) and anti-Collagen Type III antibody (#M00788-1) were obtained from Boster, and the anti-ASC antibody (#HA721306) was obtained from Hangzhou Huaan Biotechnology.

Techniques: Plasmid Preparation, Immunohistochemical staining, Staining, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay